Salivary carbonic anhydrase isoenzyme VI

HUMAN SALIVARY CARBONIC ANHYDRASE ISOENZYME VI Physiology and association with the experience of dental caries

The carbonic anhydrases (CAs) participate in the maintenance of pH homeostasis in various tissues of the human body by catalyzing the reversible reaction CO2 + H2O <=> HCO3 − + H. Carbonic anhydrase isoenzyme VI (CA VI) is secreted into the human saliva by the serous acinar cells of the parotid and submandibular glands. The present work was undertaken in order to gain an understanding of the ph...

Secretory carbonic anhydrase isoenzyme (CA VI) in human serum.

Carbonic anhydrase VI (CA VI) is a secretory isoenzyme that, by analogy to alpha-amylase, is produced in the salivary glands and delivered into saliva. To determine whether CA VI is transferred into the circulation and is detectable in human serum, we collected blood samples from four healthy subjects at 3-h intervals throughout a 24-h period and measured concentrations of CA VI by a specific t...

RELATIONSHIP BETWEEN NSAIDs, GASTRIC ANTISECRETORY AND CARBONIC ANHYDRASE ISOENZYME

Tissue and species distribution of the secreted carbonic anhydrase isoenzyme.

The secreted carbonic anhydrases, CA VI, are high molecular mass, oligomeric enzymes originally found in the sheep parotid gland and saliva. The enzymes have been purified from the saliva or parotid glands of several different species. All the CA VI enzymes studied have an apparent subunit Mr of about 45,000 as previously reported for the sheep enzyme. By Western analysis, CA VI from human, cow...

Carbonic anhydrase under pressure

Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.